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KMID : 0613820010110040379
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Journal of Life Science
2001 Volume.11 No. 4 p.379 ~ p.384
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Molecular Cloning and Phylogeny of the Human Endogenous Retrovirus HERV-W LTR Family in cDNA Library of Human Fetal Brain
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Lee Ju-Mi
Huh Jae-Won
Kim Heui-Soo
Shin Kyung-Mi
Lee Ji-Won
Lee Young-Choon
Paik In-Ho
Jang Kyung-Lib
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Abstract
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Long terminal repeats(LTRs) of the human endogenous retrovirus(HERV) heve been found to be coexpresed with genes located nearby. It has been suggested that the LTR elements have contributed to the genetic variation of human genome connected to various diseases. Recently, HERV-W family was identified in the cerebrospinal fluids and brains of individuals with schizophrenia. Using cHNA library derived from human fetal brain, we performed PCR amplification and identified seven new HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity(98∼99%) with HERV-W (AF072500). A phylogentic tree obtained by the neighbor-joining method revealed that seven new HERV-W LTR elements(FB-1, 2, 4, 8, 9, 10, 12) were closely related to the AX000960, AF072504, and AF072506 from Gen Bank database. Our data suggest that several copy numbers of the HERV-W LTR elements are expressed in human feta brain and may contribute to an understanding of biological function connected to neuropsychiatric diseases.
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KEYWORD
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HERV W, LTR elements, Human fetal brain, cDNA library, Phylogeny
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